Vogel M, Miescher S, Biaggi C, Stadler B M
Institute of Clinical Immunology, Inselspital, University of Bern, Switzerland.
Eur J Immunol. 1994 May;24(5):1200-7. doi: 10.1002/eji.1830240529.
Anti-IgE antibodies have been detected in sera of patients with an allergic disease where they might play a role in the regulation of (Ig)E-mediated reactions. Using a recombinant phage surface display expression system a combinatorial library of antibody heavy and light chains was constructed from peripheral blood mononuclear cells from an atopic donor immunized with tetanus toxoid. Screening of the library allowed the identification of a large number of phages displaying human monovalent antigen-binding fragments (Phab) against tetanus toxoid and IgE. Surprisingly, we found a high frequency of Phab against particular IgE myelomas that was comparable to the frequency found for Phab against tetanus toxoid. However, most of these Phab were directed to different idiotypic determinants, depending on the IgE myeloma used for the panning procedure. Nevertheless, two clones were found to have anti-isotypic specificity and were shown to react specifically with the CH2 domain of the IgE heavy chain.
在患有过敏性疾病的患者血清中已检测到抗IgE抗体，它们可能在（Ig）E介导的反应调节中发挥作用。利用重组噬菌体表面展示表达系统，从用破伤风类毒素免疫的特应性供体的外周血单核细胞构建了抗体重链和轻链的组合文库。对该文库的筛选使得能够鉴定出大量展示针对破伤风类毒素和IgE的人单价抗原结合片段（Phab）的噬菌体。令人惊讶的是，我们发现针对特定IgE骨髓瘤的Phab频率很高，这与针对破伤风类毒素的Phab频率相当。然而，根据用于淘选程序的IgE骨髓瘤，这些Phab中的大多数针对不同的独特型决定簇。尽管如此，发现两个克隆具有抗同种型特异性，并显示与IgE重链的CH2结构域特异性反应。
