McGregor B, Colon S, Mutin M, Chignier E, Zech P, McGregor J
Hopital Edouard Herriot, Lyon, France.
Am J Pathol. 1994 Jun;144(6):1281-7.
Extensive damage is thought to occur to endothelial cells in renal vasculitis and other glomerulopathies. The state of inflammation of these endothelial cells was investigated through the use of a panel of monoclonal antibodies (MAb) directed against thrombospondin (TSP), von Willebrand factor (vWF), integrins (alpha IIb beta 3, alpha v beta 3), CD36, and classical markers of inflammation (P-selectin, E-selectin, ICAM-1, VCAM). Results show that the anti-TSP MAb (LYP10) stains large areas of interstitium in focal sclerosis, vasculitis, membranous glomerulonephritis (GN), and diabetic GN but does not in normal kidney. In contrast, very limited areas are stained by LYP10 in minimal change nephropathy and Berger's disease. On paraffin-embedded specimens these areas stained by LYP10 appear edematous and early fibrous. Up-regulation of vWF and ICAM-1 is matched by an increased binding of LYP10 to the interstitium. In addition, fibrous crescents in injured glomeruli are stained by LYP10. This study reports for the first time an increased TSP secretion in glomerulopathies. Such TSP secretion may be part of physiological adaptive changes associated with inflammation and early fibrosis.
人们认为，肾血管炎和其他肾小球疾病中的内皮细胞会受到广泛损伤。通过使用一组针对血小板反应蛋白（TSP）、血管性血友病因子（vWF）、整合素（αIIbβ3、αvβ3）、CD36以及炎症经典标志物（P选择素、E选择素、细胞间黏附分子-1、血管细胞黏附分子）的单克隆抗体（MAb），对这些内皮细胞的炎症状态进行了研究。结果显示，抗TSP单克隆抗体（LYP10）可使局灶性硬化、血管炎、膜性肾小球肾炎（GN）和糖尿病性肾小球肾炎的间质大片区域着色，但在正常肾脏中则不会。相比之下，LYP10在微小病变性肾病和伯杰氏病中染色的区域非常有限。在石蜡包埋标本上，LYP10染色的这些区域呈现水肿和早期纤维化。vWF和细胞间黏附分子-1的上调与LYP10与间质结合增加相匹配。此外，受损肾小球中的纤维性新月体也被LYP10染色。本研究首次报道了肾小球疾病中TSP分泌增加。这种TSP分泌可能是与炎症和早期纤维化相关的生理适应性变化的一部分。
