Section of Periodontology, Faculty of Odontology, University Complutense of Madrid, Madrid, Spain.
Department of Periodontology, Universitat Internacional de Catalunya (UIC), Barcelona, Spain.
Clin Oral Investig. 2019 Feb;23(2):957-964. doi: 10.1007/s00784-018-2516-9. Epub 2018 Jun 18.
Tuberosity grafts had a greater percentage of lamina propria and lower percentage of submucosa when compared to lateral palate grafts.
The study aims to understand the differences in the structural composition of soft tissue autografts harvested from the lateral palate or the tuberosity.
Patients were randomly allocated to receive autografts harvested either from palatal or tuberosity sites to augment horizontal volume deficiencies around single-tooth implants. Tissue biopsies were analyzed for histological and histo-morphometric analysis. Picro-sirius red stain was used to evaluate collagen 1 and 3. Also, immuno-histochemical analysis was performed against MMP1, MMP2, cytokeratin-10, cytokeratin-13, and lysine hydroxylase-2.
Twenty specimens were harvested from 9 subjects in the lateral palate group (PG) and 11 subjects in the tuberosity group (TG). The percentage of lamina propria represented 51.08% in the PG group and 72.79% in the TG group, while the area of submucosa was minimal in the TG group representing 4.89% of the total sample vs 25.75% in the PG. The total area of COL-1 and 3 in the TG was 1.19 ± 0.57 and 0.72 ± 0.44 mm, respectively, while in the PG, the corresponding values were 1.4 ± 0.7 and 1.04 ± 0.5 mm. The immuno-histochemical analysis generally showed a higher expression of LLH-2, MMP2, CYT-10, and CYT-13 in the TG when compared with the PG.
Tuberosity grafts had a greater percentage of lamina propria and lower percentage of submucosa. The collagen content in the lamina propria was similar for both groups while the immuno-histochemical profile showed differences in the antibody expression of the epithelial cells.
Tuberosity grafts had more lamina propria and less submocusa, which may be beneficial for volume augmentation.
与外侧腭移植物相比，结节移植物的固有层百分比更大，而黏膜下百分比更小。
本研究旨在了解从外侧腭或结节采集的软组织自体移植物在结构组成上的差异。
将患者随机分配到接受来自腭或结节部位的自体移植物以增加单颗种植体周围水平体积不足的组中。对组织活检进行组织学和组织形态计量学分析。使用苦味酸-天狼星红染色评估胶原蛋白 1 和 3。还进行了针对 MMP1、MMP2、细胞角蛋白-10、细胞角蛋白-13 和赖氨酸羟化酶-2 的免疫组织化学分析。
在外侧腭组（PG）中从 9 名受试者中采集了 20 个标本，在结节组（TG）中从 11 名受试者中采集了 20 个标本。PG 组固有层的百分比为 51.08%，TG 组为 72.79%，而 TG 组的黏膜下面积最小，占总样本的 4.89%，而 PG 组为 25.75%。TG 中 COL-1 和 3 的总面积分别为 1.19±0.57 和 0.72±0.44mm，而在 PG 中，相应的值分别为 1.4±0.7 和 1.04±0.5mm。免疫组织化学分析通常显示 TG 中 LLH-2、MMP2、CYT-10 和 CYT-13 的表达较高，与 PG 相比。
结节移植物具有更大的固有层百分比和更小的黏膜下百分比。固有层的胶原蛋白含量在两组之间相似，而免疫组织化学谱显示上皮细胞抗体表达存在差异。
结节移植物有更多的固有层和更少的黏膜下组织，这可能有利于体积增加。
