Guan S, Marshall A G, Wahl M C
National High Magnetic Field Laboratory, Florida State University, Tallahassee 32306-4005.
Anal Chem. 1994 Apr 15;66(8):1363-7. doi: 10.1021/ac00080a024.
We present a new FT-ICR method for MS/MS of trapped ions. Parent ions isolated in the source trap of a dual-trap FT-ICR/MS instrument are subjected to off-resonance dipolar excitation to promote collision-induced dissociation (CID), while product ions are simultaneously axialized by broad-band azimuthal quadrupolar excitation in the presence of argon collision gas. In this way, radial diffusional loss of product ions is greatly reduced; moreover, the axialized product ions may then be efficiently transferred to the analyzer trap for high-resolution detection. Significant improvements in FT-ICR detection efficiency, mass resolving power (m/delta m > or = 20,000 at m/z < or = 1000), and mass accuracy (< or = 50 ppm for peptide fragments of m/z < or = 1000) are demonstrated for CID product ions of the cyclic peptide, gramicidin S.
我们提出了一种用于阱中离子串联质谱（MS/MS）的新型傅里叶变换离子回旋共振（FT-ICR）方法。在双阱FT-ICR/MS仪器的源阱中分离出的母离子受到非共振偶极激发以促进碰撞诱导解离（CID），同时在存在氩碰撞气体的情况下，产物离子通过宽带方位四极激发被同时轴向化。通过这种方式，产物离子的径向扩散损失大大降低；此外，轴向化的产物离子随后可以有效地转移到分析器阱中进行高分辨率检测。对于环肽短杆菌肽S的CID产物离子，傅里叶变换离子回旋共振检测效率、质量分辨能力（在m/z≤1000时，m/Δm≥20,000）和质量准确度（对于m/z≤1000的肽片段，≤50 ppm）都有显著提高。
