Zimmerman P A, Katholi C R, Wooten M C, Lang-Unnasch N, Unnasch T R
Department of Medicine, University of Alabama at Birmingham 35294.
Mol Biol Evol. 1994 May;11(3):384-92. doi: 10.1093/oxfordjournals.molbev.a040114.
Polymerase chain reaction (PCR) products were characterized for a repeated sequence family (designated "O-150") of the human filarial parasite Onchocerca volvulus. In phylogenetic inferences, the O-150 sequences clustered into closely related groups, suggesting that concerted evolution maintains sequence homology in this family. Using a novel mathematical model based on a nested application of an analysis of variance, we demonstrated that African rainforest and savannah strain parasite populations are significantly different. In contrast, parasites collected in the New World are indistinguishable from African savannah strains of O. volvulus. This finding supports the hypothesis that onchocerciasis was recently introduced into the New World, possibly as a result of the slave trade.
对人体丝虫寄生虫盘尾丝虫的一个重复序列家族（命名为“O - 150”）的聚合酶链反应（PCR）产物进行了特征分析。在系统发育推断中，O - 150序列聚集成密切相关的组，这表明协同进化维持了该家族中的序列同源性。使用基于方差分析嵌套应用的新型数学模型，我们证明非洲雨林和草原菌株寄生虫种群存在显著差异。相比之下，在新世界收集的寄生虫与盘尾丝虫的非洲草原菌株无法区分。这一发现支持了盘尾丝虫病最近传入新世界的假说，可能是奴隶贸易的结果。
