Besselaar T G, Blackburn N K
Department of Virology, University of the Witwatersrand, Sandringham, South Africa.
Res Virol. 1994 Jan-Feb;145(1):13-9. doi: 10.1016/s0923-2516(07)80002-1.
Monoclonal antibodies (mAb) were used to examine possible stages at which antibody-mediated neutralization of Rift Valley fever virus occurs, and to assess whether binding of antibody is dependent on viral protein structure in order that antibody recognition take place. Analysis of the structural properties of the antigenic determinants revealed that the neutralizing sites are highly conformation-dependent. None of the mAb prevented virus binding, suggesting that the epitopes they define are spatially separate from the site(s) responsible for virus attachment to the cellular receptor. The finding that many of the mAb also did not inhibit virus entry into the cell demonstrated that neutralization of RVFV infectivity by immune antibodies is not dependent on blocking at the early stages in the viral life cycle.
单克隆抗体（mAb）被用于检测抗体介导的裂谷热病毒中和作用可能发生的阶段，并评估抗体结合是否依赖于病毒蛋白结构，以便发生抗体识别。对抗抗原决定簇的结构特性分析表明，中和位点高度依赖构象。没有一种单克隆抗体能阻止病毒结合，这表明它们所定义的表位在空间上与负责病毒附着于细胞受体的位点是分开的。许多单克隆抗体也不抑制病毒进入细胞这一发现表明，免疫抗体对裂谷热病毒感染力的中和作用不依赖于在病毒生命周期早期阶段的阻断。
