Kokoskin E, Gyorkos T W, Camus A, Cedilotte L, Purtill T, Ward B
McGill University Centre for Tropical Diseases, Montreal General Hospital, Canada.
J Clin Microbiol. 1994 Apr;32(4):1074-5. doi: 10.1128/jcm.32.4.1074-1075.1994.
Changes in temperature (from room temperature to 50 degrees C) and staining time (from 90 to 10 min) were evaluated as a means of improving the detection of microsporidia from stool specimens. A blinded and independent comparison of 50 known positive matched-specimen pairs by three technologists resulted in consistently easier microscopic detection. The background is clearer, and spores stain more intensely. Staining time is reduced by 80 min.
评估了温度变化（从室温到50摄氏度）和染色时间变化（从90分钟到10分钟），以此作为改善从粪便标本中检测微孢子虫的一种方法。三位技术人员对50对已知阳性匹配标本进行了盲法独立比较，结果显示显微镜检测始终变得更容易。背景更清晰，孢子染色更强烈。染色时间减少了80分钟。
