Umemoto S, Noguchi K, Kawai Y, Senba E
Department of Anatomy and Neurobiology, Wakayama Medical College, Japan.
Brain Res Mol Brain Res. 1994 Apr;23(1-2):93-9. doi: 10.1016/0169-328x(94)90215-1.
By in situ hybridization and immunohistochemistry, we examined the expression of neuropeptides such as neuropeptide Y (NPY), galanin (Gal), substance P (SP), vasoactive intestinal polypeptide (VIP) and their mRNAs in the rat mesencephalic trigeminal nucleus (Mes5) following masseteric nerve transection. On the side contralateral to the nerve transection, none of the peptides examined were labeled in Mes5 cell bodies. However, on the side ipsilateral to the lesion, NPY, Gal and preprotachykinin (PPT) mRNAs appeared in Mes5 cell bodies. Double labeling for mRNAs by in situ hybridization and retrograde tracer fluoro-gold (FG) revealed that almost all (96-97%) the FG-labeled neurons which were cut expressed NPY and Gal mRNAs, whereas less neurons (87%) expressed PPT mRNA. NPY and Gal-like immunoreactivities were detected in Mes5 cell bodies ipsilateral to the axotomy. The results suggested that these neuropeptides play roles in adaptive processes after peripheral nerve injury in Mes5 neurons as they are thought to do so in dorsal root ganglion neurons.
通过原位杂交和免疫组织化学方法，我们检测了大鼠咬肌神经横断后，中脑三叉神经核（Mes5）中神经肽Y（NPY）、甘丙肽（Gal）、P物质（SP）、血管活性肠肽（VIP）及其mRNA的表达。在神经横断对侧，Mes5细胞体中未检测到所研究的任何一种肽的标记。然而，在损伤同侧，Mes5细胞体中出现了NPY、Gal和前速激肽原（PPT）mRNA。通过原位杂交和逆行示踪剂荧光金（FG）对mRNA进行双重标记显示，几乎所有（96 - 97%）被切断的FG标记神经元都表达NPY和Gal mRNA，而表达PPT mRNA的神经元较少（87%）。在轴突切断同侧的Mes5细胞体中检测到NPY和Gal样免疫反应性。结果表明，这些神经肽在Mes5神经元外周神经损伤后的适应性过程中发挥作用，正如它们在背根神经节神经元中被认为的那样。
