Argémi B, Hours M C, Even F, Garguilo G, Hollard J M
Acta Endocrinol (Copenh). 1978 May;88(1):75-86. doi: 10.1530/acta.0.0880075.
Evaluations of human immunoreactive calcitonin (IRCT) assay have been extensively reviewed. Labelled hormone was re-purified on carboxymethyl-cellulose in order to isolate a fraction containing mainly monoiodinated calcitonin, which was found to be very stable. Two antisera with different immunochemical characteristics were used for incubation studies, one of which was incubated with unextracted and extracted plasma samples. The sensitivity of the assays was 60 pg/ml plasma. However, marked differences were observed in the results obtained by the three methods depending on the importance of the inhibitory effect of plasma on the binding of the tracer to antibodies. However, the absolute plasma IRCT level could not be related to the presence of calcitonin M, except in one case. Further studies are needed in order to ascertain the origin and the significance of the immunoreactive material which was detected in normal plasma by one antiserum.
对人免疫反应性降钙素（IRCT）检测方法的评估已得到广泛综述。标记激素在羧甲基纤维素上重新纯化，以分离出主要含单碘化降钙素的部分，发现其非常稳定。使用两种具有不同免疫化学特性的抗血清进行孵育研究，其中一种与未提取和提取的血浆样本孵育。检测方法的灵敏度为血浆60 pg/ml。然而，根据血浆对示踪剂与抗体结合的抑制作用的重要性，三种方法获得的结果存在显著差异。然而，除了一个病例外，血浆IRCT的绝对水平与降钙素M的存在无关。需要进一步研究以确定一种抗血清在正常血浆中检测到的免疫反应性物质的来源和意义。
