Sainte-Laudy J, Vallon C, Guérin J C
Unité d'Immuno-allergologie, laboratoire Cerba, Val d'Oise.
Allerg Immunol (Paris). 1996 Feb;28(2):44-7.
Pharmacological or immunological activation of human basophil induces the expression of membrane markers such as CD63 and the release of histamine and leukotriene C4 (LTC4). LTC4 release test has the advantages of a cellular test which is obviously closer to the physio-pathological reaction than the direct determination of circulating antibodies. Moreover, the determination of a newly formed mediator eliminates the spontaneous release of mediator by simple cellular damage. We present here, after having controlled the reliability of the method for aero-allergens, preliminary results of LTC4 release obtained for haptens (ampicillin, methylprednisolone and acetylsalicylic acid) which are in favour of the use of this method in the complex field of drug allergy diagnosis.
人嗜碱性粒细胞的药理或免疫激活可诱导膜标志物如CD63的表达以及组胺和白三烯C4（LTC4）的释放。LTC4释放试验具有细胞试验的优势，该试验明显比直接测定循环抗体更接近生理病理反应。此外，新形成介质的测定消除了因简单细胞损伤导致的介质自发释放。在控制了该方法对空气变应原的可靠性后，我们在此展示了对半抗原（氨苄青霉素、甲基泼尼松龙和乙酰水杨酸）获得的LTC4释放的初步结果，这些结果支持在药物过敏诊断的复杂领域中使用该方法。
