Vieths S, Jankiewicz A, Schöning B, Aulepp H
Institute of Food Chemistry, Technical University Berlin, Germany.
Allergy. 1994 Apr;49(4):262-71. doi: 10.1111/j.1398-9995.1994.tb02659.x.
Low-temperature, acetone powder extracts were prepared from mature fruit of 16 apple strains. SDS-PAGE and immunoblot analysis revealed great variation in the relative amounts of the 18-kDa apple allergen in these extracts. EAST (RAST) scores, measured with individual and pool sera from patients allergic to birch pollen and apples, ranged from 0.2 to 4.0 and were related to the relative amount of the 18-kDa protein. These findings were confirmed by ELISA-inhibition assays, dose-related histamine release, semiquantitative evaluation of immunoblots by absorption/reflection densitometry, and skin prick tests with extracts of Golden Delicious, Boskoop, and Jamba apples (corresponding to a high, low, and very low 18-kDa allergen content). Additional open oral challenge tests were performed with two apple-allergic patients and 15 and 16 apple strains. With all methods, the deduced allergenic potency decreased in the following order: Golden Delicious > Boskoop > Jamba. Therefore, we concluded that the IgE-binding potency of apple strains depends on the occurrence of the 18-kDa allergen.
从16个苹果品种的成熟果实中制备了低温丙酮粉提取物。SDS-PAGE和免疫印迹分析显示，这些提取物中18 kDa苹果过敏原的相对含量存在很大差异。用对桦树花粉和苹果过敏患者的个体血清和混合血清测量的EAST（RAST）评分范围为0.2至4.0，且与18 kDa蛋白的相对含量相关。这些发现通过ELISA抑制试验、剂量相关的组胺释放、通过吸收/反射密度测定法对免疫印迹进行半定量评估以及用金冠、博斯科普和詹巴苹果提取物进行皮肤点刺试验得到了证实（分别对应高、低和极低的18 kDa过敏原含量）。另外对两名苹果过敏患者以及15个和16个苹果品种进行了开放性口服激发试验。使用所有方法得出的致敏效力按以下顺序降低：金冠>博斯科普>詹巴。因此，我们得出结论，苹果品种的IgE结合效力取决于18 kDa过敏原的存在情况。
