Brown S C, Thomson S A, Veal J M, Davis D G
Glaxo Research Institute, Research Triangle Park, NC 27709.
Science. 1994 Aug 5;265(5173):777-80. doi: 10.1126/science.7519361.
Peptide nucleic acids (PNA) incorporating nucleic acid bases into an achiral polyamide backbone bind to DNA in a sequence-dependent manner. The structure of a PNA-ribonucleic acid (RNA) complex was determined with nuclear magnetic resonance methods. A hexameric PNA formed a 1:1 complex with a complementary RNA that is an antiparallel, right-handed double helix with Watson-Crick base pairing similar to the "A" form structure of RNA duplexes. The achiral PNA backbone assumed a distinct conformation upon binding that differed from previously proposed models and provides a basis for further structure-based design of antisense agents.
将核酸碱基掺入非手性聚酰胺主链的肽核酸（PNA）以序列依赖的方式与DNA结合。采用核磁共振方法确定了PNA-核糖核酸（RNA）复合物的结构。一个六聚体PNA与一条互补RNA形成1:1复合物，该复合物是一个反平行的右手双螺旋结构，具有与RNA双链体“A”型结构相似的沃森-克里克碱基配对。非手性PNA主链在结合时呈现出一种独特的构象，这与先前提出的模型不同，并为基于结构的反义药物进一步设计提供了基础。
