Murray J B, Collier A K, Arnold J R
Department of Genetics, University of Leeds, United Kingdom.
Anal Biochem. 1994 Apr;218(1):177-84. doi: 10.1006/abio.1994.1157.
A general procedure for the purification of chemically synthesized oligoribonucleotides is reported. Purification based on the use of a single reverse-phase HPLC column with buffer systems of differing ion-pairing capacity is described. These methods have been applied to the preparation of a series of RNAs which range in size from 10 to 46 nucleotides. The yields obtained are high, up to 53% (based on isolated product compared to those obtained from final trityl assay). The purity of the isolated material is 96-99%. Thus with this general procedure, milligram quantities of extremely pure RNA can be efficiently obtained.
报道了一种化学合成寡核糖核苷酸的通用纯化方法。描述了基于使用具有不同离子配对能力缓冲系统的单一反相高效液相色谱柱进行纯化的方法。这些方法已应用于制备一系列大小从10到46个核苷酸的RNA。获得的产率很高，高达53%（基于分离产物与最终三苯甲基测定法获得的产物相比）。分离材料的纯度为96-99%。因此，通过这种通用方法，可以高效地获得毫克量的极纯RNA。
