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Mechanism of inactivation of Escherichia coli and Lactobacillus leichmannii ribonucleotide reductases by 2'-chloro-2'-deoxynucleotides: evidence for generation of 2-methylene-3(2H)-furanone.2'-氯-2'-脱氧核苷酸使大肠杆菌和莱氏乳杆菌核糖核苷酸还原酶失活的机制：生成2-亚甲基-3(2H)-呋喃酮的证据
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Enzymatic and NMR analysis of oligoribonucleotides synthesized with 2'-tert-butyldimethylsilyl protected cyanoethylphosphoramidite monomers.对用2'-叔丁基二甲基甲硅烷基保护的氰基乙基亚磷酰胺单体合成的寡核糖核苷酸进行酶促和核磁共振分析。
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Deuterium NMR investigation of backbone dynamics in the synthetic oligonucleotide [d(CGCGAATTCGCG)]2.合成寡核苷酸[d(CGCGAATTCGCG)]₂主链动力学的氘核磁共振研究。
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High-resolution NMR studies of chimeric DNA-RNA-DNA duplexes, heteronomous base pairing, and continuous base stacking at junctions.嵌合DNA-RNA-DNA双链体、异源碱基配对以及连接处连续碱基堆积的高分辨率核磁共振研究。
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骨架氘标记的[r(CGCGAAUUCGCG)]2的合成及合成RNA的高效液相色谱纯化

Synthesis of backbone deuterium labelled [r(CGCGAAUUCGCG)]2 and HPLC purification of synthetic RNA.

作者信息

Khare D, Orban J

机构信息

Maryland Biotechnology Institute, University of Maryland, Rockville 20850.

出版信息

Nucleic Acids Res. 1992 Oct 11;20(19):5131-6. doi: 10.1093/nar/20.19.5131.

DOI:10.1093/nar/20.19.5131
PMID:1383940
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC334295/
Abstract

The chemical synthesis of backbone deuterium labelled [r(CGCGAAUUCGCG)]2 (U* = [5'-2H]U) is described. An efficient purification procedure was developed using a polymeric reverse phase (PRP) HPLC column at 60 degrees C. This procedure provided pure RNA dodecamer in the multi-milligram quantities (39% overall yield) necessary for dynamics studies using solid-state deuterium NMR. The purification method has been effectively applied to other RNA sequences and will assist biophysical studies which require relatively large quantities of RNA oligomers.

摘要

描述了主链氘标记的[r(CGCGAAUUCGCG)]2（U* = [5'-2H]U）的化学合成。开发了一种在60℃下使用聚合物反相（PRP）HPLC柱的高效纯化方法。该方法以多毫克量（总产率39%）提供了用于固态氘核磁共振动力学研究所需的纯RNA十二聚体。该纯化方法已有效地应用于其他RNA序列，并将有助于需要相对大量RNA寡聚物的生物物理研究。