Yen T T, Greenberg M M, Yu P L, Pearson D V
Horm Metab Res. 1976 May;8(3):159-66. doi: 10.1055/s-0028-1093672.
The development of obesity, hyperinsulinemia and six hepatic lipogenic enzymes in Avy/a mice were compared to that in a/a mice. Correlation between body weight, liver weight, plasma insulin concentration and activities of hepatic enzymes was analyzed. In the Avy/a mice, body weight, liver weight and plasma insulin level increased steadily as the mice aged. In the a/a mice, the change of these three parameters was much slower. Plasma insulin concentration in a/a mice did not increase until eight months of age. Compared with a/a mice, Avy/a mice had higher 6-phosphogluconate dehydrogenase and fatty acid synthetase activities at two months of age; lower citrate cleavage enzyme, glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase activities at three months of age; lower citrate cleavage enzyme and glucose-6-phosphate dehydrogenase and higher acetyl CoA carboxylase activities at five months of age; and higher malic enzyme, citrate cleavage enzyme and 6-phosphogluconate dehydrogenase activities at eight months of age. There were significant correlations between plasma insulin level and body weight and between plasma insulin level and the activities of malic enzyme and citrate cleavage enzyme in Avy/a mice. The correlation between body weight and malic enzyme and citrate cleavage enzyme activities disappeared after the analysis was adjusted for plasma insulin level.
将Avy/a小鼠与a/a小鼠的肥胖、高胰岛素血症及六种肝脏脂肪生成酶的发育情况进行了比较。分析了体重、肝脏重量、血浆胰岛素浓度与肝脏酶活性之间的相关性。在Avy/a小鼠中，随着小鼠年龄增长，体重、肝脏重量和血浆胰岛素水平稳步上升。在a/a小鼠中，这三个参数的变化要慢得多。a/a小鼠的血浆胰岛素浓度直到八个月龄时才升高。与a/a小鼠相比，Avy/a小鼠在两个月龄时6-磷酸葡萄糖酸脱氢酶和脂肪酸合成酶活性较高；在三个月龄时柠檬酸裂解酶、葡萄糖-6-磷酸脱氢酶和6-磷酸葡萄糖酸脱氢酶活性较低；在五个月龄时柠檬酸裂解酶和葡萄糖-6-磷酸脱氢酶较低而乙酰辅酶A羧化酶活性较高；在八个月龄时苹果酸酶、柠檬酸裂解酶和6-磷酸葡萄糖酸脱氢酶活性较高。在Avy/a小鼠中，血浆胰岛素水平与体重之间以及血浆胰岛素水平与苹果酸酶和柠檬酸裂解酶活性之间存在显著相关性。在对分析结果进行血浆胰岛素水平校正后，体重与苹果酸酶和柠檬酸裂解酶活性之间的相关性消失。
