Sebbelov A M, Svendsen C, Jensen H, Kjaer S K, Norrild B
DNA Tumor Virus Laboratory, Institute of Medical Microbiology, University of Copenhagen.
Res Virol. 1994 Mar-Apr;145(2):83-92. doi: 10.1016/s0923-2516(07)80010-0.
Archival formalin-fixed paraffin-embedded cervical specimens from 125 women in Greenland and 139 women in Denmark who had CIN I-III or cervical cancer diagnosed between 1983 and 1987 were analysed for human papillomavirus type 16 (HPV-16) by in situ hybridization and for HPV-16, 18, 31, 33, 35 and 45 by PCR. In situ hybridization analysis showed an HPV-16 prevalence of 17% (16/95) and 23% (24/105) in the premalignant lesions from Greenland and Denmark, respectively. The HPV-16 prevalence rate in the cancer specimens was 10% (3/30) in the samples from Greenland and 29% (10/34) in the Danish specimens. A total of 82 Greenlandic and 107 Danish specimens were beta-globin-positive by PCR reaction. HPV-16-specific PCR on these samples showed 63% (34/54) of the Greenlandic and 68% (50/74) of the Danish preinvasive lesions to be positive. The corresponding HPV-16-positive rates for the invasive cancers were 82% (23/28) for Greenland and 70% (23/33) for Denmark. This study of patient samples supports our previous population-based studies in which similar HPV-detection rates were found among random samples of women from Greenland and Denmark, although Greenland has a 4-5-fold higher cervical cancer incidence.
对1983年至1987年间被诊断为CIN I - III级或宫颈癌的125名格陵兰女性和139名丹麦女性的存档福尔马林固定石蜡包埋宫颈标本进行分析，通过原位杂交检测16型人乳头瘤病毒（HPV - 16），并通过聚合酶链反应（PCR）检测HPV - 16、18、31、33、35和45型。原位杂交分析显示，格陵兰和丹麦的癌前病变中HPV - 16的患病率分别为17%（16/95）和23%（24/105）。格陵兰样本中癌标本的HPV - 16患病率为10%（3/30），丹麦标本中为29%（10/34）。通过PCR反应，共有82份格陵兰标本和107份丹麦标本的β - 珠蛋白呈阳性。对这些样本进行的HPV - 16特异性PCR显示，格陵兰63%（34/54）的癌前病变和丹麦68%（50/74）的癌前病变呈阳性。格陵兰浸润性癌的相应HPV - 16阳性率为82%（23/28），丹麦为70%（23/33）。对患者样本的这项研究支持了我们之前基于人群的研究，在该研究中，尽管格陵兰宫颈癌发病率高出4至5倍，但在格陵兰和丹麦女性的随机样本中发现了相似的HPV检测率。
