Zhou J H, Hikono H, Ohtaki M, Kubota T, Sakurai M
Institute of Society for Techno-innovation of Agriculture, Forestry and Fisheries, Ibaraki, Japan.
Biochim Biophys Acta. 1994 Aug 11;1223(1):148-50. doi: 10.1016/0167-4889(94)90084-1.
The cDNA clones encoding two isoforms of bovine stem cell factor (bSCF) were obtained using reverse transcriptase-polymerase chain reaction, and their sequences were determined. The deduced amino acid sequences of the longer and shorter isoforms of bSCF consist, respectively, of 274 and 246 residues and show a high degree of identity to those of SCFs of different animal species. Northern blot analysis with the cDNA revealed the expression of a 5.8 kilobase bSCF RNA in fetal bovine tissues.
利用逆转录聚合酶链反应获得了编码牛干细胞因子（bSCF）两种同工型的cDNA克隆，并测定了它们的序列。bSCF较长和较短同工型的推导氨基酸序列分别由274个和246个残基组成，与不同动物物种的SCF具有高度同源性。用该cDNA进行的Northern印迹分析显示，在胎牛组织中存在5.8千碱基的bSCF RNA表达。
