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编码猫干细胞因子两种同工型的cDNA的克隆与测序

The cloning and sequencing of cDNAs encoding two isoforms of feline stem cell factor.

作者信息

Dunham S P, Onions D E

机构信息

Department of Veterinary Pathology, University of Glasgow Veterinary School, U.K.

出版信息

DNA Seq. 1996;6(4):233-7. doi: 10.3109/10425179609008448.

DOI:10.3109/10425179609008448
PMID:8912926
Abstract

cDNA clones encoding two isoforms of feline stem cell factor (fSCF) have been isolated using RT-PCR and their sequences determined. The cDNAs encode a predicted full length fSCF protein of 274 amino-acids and a shorter isoform of 246 amino acids. Feline SCF shows a high degree of homology to the SCFs of other species at both the nucleic acid and protein level.

摘要

利用逆转录聚合酶链反应(RT-PCR)分离出了编码猫干细胞因子(fSCF)两种亚型的cDNA克隆,并测定了它们的序列。这些cDNA编码一个预测的全长为274个氨基酸的fSCF蛋白和一个较短的246个氨基酸的亚型。猫SCF在核酸和蛋白质水平上与其他物种的SCF具有高度同源性。

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