Petitte J N, Kulik M J
Department of Poultry Science, North Carolina State University, Raleigh 27695-7608, USA.
Biochim Biophys Acta. 1996 Jun 7;1307(2):149-51. doi: 10.1016/0167-4781(96)00062-0.
Stem cell factor (SCF), also known as Steel factor, is a transmembrane cytokine involved in several developmental processes and the ligand for the receptor tyrosine kinase c-kit. In several mammalian species, two isoforms of stem cell factor have been reported, a long form in which soluble SCF is released after proteolytic cleavage of the extracellular domain and a short membrane-anchored form in which a region containing a cleavage site is deleted. Currently, only the longer, soluble form has been identified in birds. Therefore, the cDNAs encoding two forms of quail stem cell factor (qSCF) were obtained using RT-PCR with nested primers. The deduced amino acid sequence of the long form of qSCF showed a high degree of homology with chicken (98%) and relatively low homology (approximately 53%) with various mammalian SCFs. Northern blot analysis with the qSCF cDNA revealed the expression of a 5.9 and a 2.7 kb transcript in several quail tissues.
干细胞因子（SCF），也被称为Steel因子，是一种跨膜细胞因子，参与多个发育过程，是受体酪氨酸激酶c-kit的配体。在几种哺乳动物中，已报道了干细胞因子的两种异构体，一种是长形式，其细胞外结构域经蛋白水解切割后可释放可溶性SCF；另一种是短的膜锚定形式，其中包含切割位点的区域被删除。目前，在鸟类中仅鉴定出了较长的可溶性形式。因此，使用巢式引物通过RT-PCR获得了编码鹌鹑干细胞因子（qSCF）两种形式的cDNA。qSCF长形式推导的氨基酸序列与鸡具有高度同源性（98%），与各种哺乳动物的SCF同源性相对较低（约53%）。用qSCF cDNA进行的Northern印迹分析显示，在几种鹌鹑组织中表达了5.9 kb和2.7 kb的转录本。
