Miyahara T, Shiozawa S, Murai A
J Gerontol. 1978 Jul;33(4):498-503. doi: 10.1093/geronj/33.4.498.
To study the effect of age on amino acid composition of human skin collagen, skins from 8 women aged 0 to 93 were extracted with acetone and EDTA and then gelatinized. Gelatin was purified by extraction with chloroform-methanol and DEAE cellulose chromatography. Hydroxyproline was spectrophotometrically determined and its contents were essentially the same (12.6%). Amino acid analysis indicated no significant age-related variations in the contents of proline, hydroxyproline, lysine, and hydroxylysine over the range of 0 to 93 years of age. The content of cysteine was reasonably low (0.4 to 0.6 per 1,000 amino acid residues) except in the cases of those 0 and 93 years of age. Aldehyde content was slightly lower in young cases than in infant and old cases. These results indicate that the changes in cross-links derived from aldehyde may be responsible for the effect of age.
为研究年龄对人皮肤胶原蛋白氨基酸组成的影响，对8名年龄在0至93岁的女性皮肤用丙酮和乙二胺四乙酸进行提取，然后使其明胶化。通过氯仿 - 甲醇提取和二乙氨基乙基纤维素色谱法对明胶进行纯化。用分光光度法测定羟脯氨酸，其含量基本相同（12.6%）。氨基酸分析表明，在0至93岁范围内，脯氨酸、羟脯氨酸、赖氨酸和羟赖氨酸的含量没有明显的年龄相关变化。半胱氨酸含量相对较低（每1000个氨基酸残基含0.4至0.6个），0岁和93岁的情况除外。年轻个体的醛含量略低于婴儿和老年个体。这些结果表明，源自醛的交联变化可能是年龄效应的原因。
