Finkelstein I, Trope G E, Basu P K, Hasany S M, Hunter W S
Department of Ophthalmology, University of Toronto, Ontario, Canada.
Br J Ophthalmol. 1990 May;74(5):280-2. doi: 10.1136/bjo.74.5.280.
The purpose of this study was to compare collagen content in the TM of normal and glaucomatous eyes, and to establish whether collagen levels change with age. Collagen content was measured in 30 normal and 27 age matched glaucoma trabeculectomy specimens by the sirius red dye binding technique, and in 14 normal and 15 age matched glaucoma specimens by amino acid analysis. Both dye binding data and amino acid analysis showed no statistical difference between normal and glaucoma samples. Age had no significant effect on mean optical densities or on the collagen-specific amino acids proline, hydroxyproline, and hydroxylysine. Amino acid variability, however, was statistically different between the two groups. These results indicate that mean collagen levels in the trabecular meshwork of glaucomatous eyes do not differ from those in normal eyes.
本研究的目的是比较正常眼和青光眼眼中小梁网的胶原蛋白含量，并确定胶原蛋白水平是否随年龄变化。通过天狼星红染料结合技术，对30个正常小梁切除术标本和27个年龄匹配的青光眼小梁切除术标本的胶原蛋白含量进行了测量；通过氨基酸分析，对14个正常标本和15个年龄匹配的青光眼标本的胶原蛋白含量进行了测量。染料结合数据和氨基酸分析均显示，正常样本和青光眼样本之间无统计学差异。年龄对平均光密度或胶原蛋白特异性氨基酸脯氨酸、羟脯氨酸和羟赖氨酸均无显著影响。然而，两组之间的氨基酸变异性存在统计学差异。这些结果表明，青光眼眼小梁网中的平均胶原蛋白水平与正常眼并无差异。
