Tournaye H, Janssens R, Verheyen G, Devroey P, Van Steirteghem A
Centre for Reproductive Medicine, University Hospital, Brussels Free University, Belgium.
Fertil Steril. 1994 Sep;62(3):574-9. doi: 10.1016/s0015-0282(16)56948-9.
To evaluate whether incubation of spermatozoa with both pentoxifylline and 2-deoxyadenosine would improve fertilization rates in couples with previous IVF failure.
Autocontrolled design in which sibling oocytes were inseminated at random in vitro with spermatozoa treated or not treated by pentoxifylline and 2-deoxyadenosine.
Oocyte quality, sperm motility, fertilization in vitro, and embryo quality.
Sperm motility was found optimized by metabolic stimulation using pentoxifylline and 2-deoxyadenosine. The mean fertilization rate per patient was 33.1% in the treatment group compared with 37.0% in the control group. The mean cleavage rate per patient was 79.6% for treatment versus 68.7% for control embryos. No differences in embryo quality were noted.
The results of this study demonstrate that an indiscriminate use of pentoxifylline and 2-deoxyadenosine is not beneficial to fertilization in couples with previous IVF failure. Further prospective research may be needed to assess the benefit of pentoxifylline and 2-deoxyadenosine in patients selected by preliminary functional in vitro tests.
评估己酮可可碱和2-脱氧腺苷共同孵育精子是否能提高既往体外受精失败夫妇的受精率。
自身对照设计，即对同胞卵母细胞进行体外随机授精，所用精子经或未经己酮可可碱和2-脱氧腺苷处理。
卵母细胞质量、精子活力、体外受精情况及胚胎质量。
发现使用己酮可可碱和2-脱氧腺苷进行代谢刺激可优化精子活力。治疗组每位患者的平均受精率为33.1%，而对照组为37.0%。治疗组胚胎的每位患者平均卵裂率为79.6%，对照组为68.7%。未观察到胚胎质量存在差异。
本研究结果表明，对于既往体外受精失败的夫妇，不加区分地使用己酮可可碱和2-脱氧腺苷对受精并无益处。可能需要进一步的前瞻性研究来评估己酮可可碱和2-脱氧腺苷对通过初步体外功能测试筛选出的患者的益处。
