用苯并咪唑衍生物对真菌细胞核进行荧光染色。

Fluorescent staining of fungal nuclei with a benzimidazol derivative.

作者信息

Lemke P A, Kugleman B, Morimoto H, Jacobs E C, Ellison J

出版信息

J Cell Sci. 1978 Feb;29:77-84. doi: 10.1242/jcs.29.1.77.

DOI:10.1242/jcs.29.1.77

Abstract

A direct staining procedure is described for fluorescence microscopy of fungal nuclei, chromosomes and mitochondria. The fluorochrome is a benzimidazol derivative (33258 Hoechst) known to bind selectively to deoxyribonucleic acid at neutral pH. The advantages of 33258 Hoechst relative to Feulgen compounds used previously to stain these structures include a greater intensity of fluorescence, the absence of fading or rapid quenching of the fluorescence, and the omission of acid hydrolysis from the procedure for removal of ribonucleic acid. 33258 Hoechst has been evaluated as a nuclear stain with a yeast (Saccharomyces cerevisiae) and a filamentous fungus (Agaricus bisporus) and appears to penetrate easily vegetative cells and spores of both fungi.

摘要

本文描述了一种用于真菌细胞核、染色体和线粒体荧光显微镜观察的直接染色方法。荧光染料是一种苯并咪唑衍生物（33258 Hoechst），已知在中性pH条件下能选择性地与脱氧核糖核酸结合。相对于先前用于染色这些结构的福尔根化合物，33258 Hoechst的优点包括荧光强度更高、荧光不褪色或快速淬灭，以及在去除核糖核酸的过程中无需进行酸水解。33258 Hoechst已被评估为一种核染色剂，用于酵母（酿酒酵母）和丝状真菌（双孢蘑菇），似乎能轻易穿透这两种真菌的营养细胞和孢子。