Lombard-Vignon N, Desplechain C, Egloff H, Franck F, Goujon N
Institut d'Anatomie et de Cytologie Pathologiques, Parc Technologique la Pardieu, Clermont-Ferrand.
Ann Pathol. 1994;14(4):255-6.
A domestic microwave oven was used to modify the Feulgen staining procedure in order to speed up the preparation of cytopathologic smears for the quantification of cell DNA in image analysis. This simple technique needs some precautions concerning the temperature, the hydrolysis time and the position of staining dishes inside the oven must be taken in order to get reproducible results. Thereafter, the conventional staining time can be reduced to 30%. This technique can be applied to cytopathologic smears from various tissues: bladder, prostate, breast, ovary.
使用家用微波炉对福尔根染色程序进行改良，以加快细胞病理学涂片的制备，用于图像分析中细胞DNA的定量。为获得可重复的结果，这项简单技术在温度、水解时间以及染色皿在烤箱内的位置方面需要一些注意事项。此后，传统染色时间可缩短至30%。该技术可应用于来自各种组织的细胞病理学涂片：膀胱、前列腺、乳腺、卵巢。
