Vennix P P, Kuijpers W, Peters T A, Tonnaer E L, Ramaekers F C
Department of Otorhinolaryngology, University of Nijmegen, The Netherlands.
Arch Otolaryngol Head Neck Surg. 1994 Oct;120(10):1102-11. doi: 10.1001/archotol.1994.01880340046008.
To determine the behavior of epidermal cells after transplantation in the middle ear.
In a rat model, full-thickness meatal skin grafts were transplanted into the middle ear and studied morphologically and immunohistochemically with the use of antibodies directed against different cytokeratin (Ck) polypeptides, which are markers of different types of epithelial cell differentiation.
The grafts had either transformed into epithelial cysts or had become integrated into the middle ear epithelium. The epithelium of the integrated grafts showed gradual transition into the epithelium of the middle ear. A clear distinction between epidermal cells and middle ear epithelium could be made only on the basis of their Ck profiles. The Ck profiles of the grafts revealed a decrease in the expression of epidermal Cks, while nonepidermal Cks became expressed. These changes can be ascribed to replacement of the dermal mesenchyma by mesenchyma from the middle ear. In two ears with superimposed infection, the graft epithelium showed expansive growth.
Meatal epidermis is well tolerated in the middle ear, but superimposed infection can induce expansive growth. These findings favor the concept that the progressive growth of cholesteatoma is related to the presence of inflammatory processes.
确定表皮细胞移植至中耳后的行为表现。
在大鼠模型中，将全层耳道皮肤移植物移植到中耳，并使用针对不同细胞角蛋白（Ck）多肽的抗体进行形态学和免疫组织化学研究，这些多肽是不同类型上皮细胞分化的标志物。
移植物要么转化为上皮囊肿，要么已整合到中耳上皮中。整合移植物的上皮显示出逐渐过渡到中耳上皮。只有根据其Ck谱才能明确区分表皮细胞和中耳上皮。移植物的Ck谱显示表皮Ck的表达减少，而非表皮Ck开始表达。这些变化可归因于中耳间充质替代了真皮间充质。在两只伴有叠加感染的耳朵中，移植物上皮显示出扩张性生长。
耳道表皮在中耳中耐受性良好，但叠加感染可诱导扩张性生长。这些发现支持胆脂瘤的进行性生长与炎症过程的存在有关这一概念。
