Zółtowska A
Department of Immunopathology, Medical Academy, Gdańsk, Poland.
Arch Immunol Ther Exp (Warsz). 1995;43(1):15-22.
In this study coexpression of cytokeratin and desmin, and occasionally also Ki-1 antigen, was displayed in extrafollicular reticulum cells of reactive lymph nodes. The absence or expression of trace amounts of these proteins in normal lymphoid tissue suggests that activation of T cell regions is correlated with the increased frequency of cytokeratin, desmin and Ki-1 expressing cells, and therefore may be a transient phenomenon. S-100-positive interdigitating reticular cells were found occasionally in extrafollicular T cell region of normal lymph nodes. They were, however, more numerous in reactive lymphatic tissue. In the myasthenic thymuses cells forming Hassall's corpuscles displayed coexpression of cytokeratin, desmin and Ki-1 antigen. Medullary epithelial cells were also cytokeratin-positive and, additionally, Ki-1 antigen was expressed on some cells dispersed in whole thymic tissue. S-100-positive interdigitating reticular cells were especially numerous in the thymic medulla and some of them found inside the Hassall's corpuscles. In Hodgkin's disease deficiency of cytokeratin and desmin in extrafollicular reticulum cells is a constant phenomenon in spite of a classic inflammatory background. However, Ki-1 antigen displayed Reed-Sternberg cells which, similar as some thymic cell elements, appear to originate from stromal perivascular mesenchyme. This fact suggests that Reed-Sternberg cells in Hodgkin's disease are pathologic counterparts of extrafollicular reticulum cells which represent a cellular differentiation defect to produce desmin and cytokeratin but with a possibility of Ki-1 antigen expression. The consequence of this may be the disregulation of immune system and the observed immunologic abnormalities. Further studies are needed to elucidate the role of Epstein-Barr viruses in this process. S-100-positive interdigitating reticular cells were in close contact with Reed-Sternberg cells and they were especially large and with numerous cells processes in the mixed cellularity (MC) subtype. The occurrence of interdigitating reticulum cells with S-100 protein expression, especially numerous in the T cell region activated of peripheral lymphatic tissue, as well as their close contact with Reed-Sternberg cells and with cells forming Hassall's corpuscles suggest their eventual possible role in the function of the immune system.
在本研究中，反应性淋巴结的滤泡外网状细胞显示出细胞角蛋白和结蛋白的共表达，偶尔还显示Ki-1抗原的表达。这些蛋白质在正常淋巴组织中的缺失或微量表达表明，T细胞区域的激活与细胞角蛋白、结蛋白和表达Ki-1的细胞频率增加相关，因此可能是一种短暂现象。在正常淋巴结的滤泡外T细胞区域偶尔发现S-100阳性的指状网状细胞。然而，它们在反应性淋巴组织中更为常见。在重症肌无力患者的胸腺中，形成哈氏小体的细胞显示出细胞角蛋白、结蛋白和Ki-1抗原的共表达。髓质上皮细胞也呈细胞角蛋白阳性，此外，一些分散在整个胸腺组织中的细胞表达Ki-1抗原。S-100阳性的指状网状细胞在胸腺髓质中特别多，其中一些位于哈氏小体内。在霍奇金淋巴瘤中，尽管有典型的炎症背景，但滤泡外网状细胞中细胞角蛋白和结蛋白的缺乏是一种持续现象。然而，Ki-1抗原在里德-斯腾伯格细胞中表达，这些细胞与一些胸腺细胞成分相似，似乎起源于血管周围的基质间充质。这一事实表明，霍奇金淋巴瘤中的里德-斯腾伯格细胞是滤泡外网状细胞的病理对应物，滤泡外网状细胞存在产生结蛋白和细胞角蛋白的细胞分化缺陷，但有可能表达Ki-1抗原。其结果可能是免疫系统失调以及观察到的免疫异常。需要进一步研究以阐明爱泼斯坦-巴尔病毒在此过程中的作用。S-100阳性的指状网状细胞与里德-斯腾伯格细胞密切接触，在混合细胞型（MC）亚型中它们特别大且有许多细胞突起。表达S-100蛋白的指状网状细胞的出现，在周围淋巴组织激活的T细胞区域特别多，以及它们与里德-斯腾伯格细胞和形成哈氏小体的细胞的密切接触，表明它们最终可能在免疫系统功能中发挥作用。
