超氧阴离子诱导大鼠腹膜肥大细胞释放组胺。

Superoxide anion-induced histamine release from rat peritoneal mast cells.

作者信息

Akagi M, Katakuse Y, Fukuishi N, Kan T, Akagi R

机构信息

Department of Pharmacology, Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Japan.

出版信息

Biol Pharm Bull. 1994 May;17(5):732-4. doi: 10.1248/bpb.17.732.

DOI:10.1248/bpb.17.732

PMID:7522732

Abstract

We investigated the properties of superoxide anion (O2-)-induced histamine release from rat peritoneal mast cells using superoxide anion radicals obtained from potassium superoxide (KO2). KO2 elicited a rapid histamine release in a dose-dependent fashion, without lactate dehydrogenase (LDH) release. The KO2-induced release was temperature- and energy-dependent. KO2 rapidly increased the intracellular free Ca2+ concentration, accompanied by a marked increase of Ca(2+)-uptake. These findings indicate that the increase in intracellular Ca2+ concentration is involved in the initiation of KO2-induced histamine release, and KO2 could be used as an agent of O2(-)-induced biological reactions.

摘要

我们使用超氧化钾（KO₂）产生的超氧阴离子自由基，研究了超氧阴离子（O₂⁻）诱导大鼠腹膜肥大细胞释放组胺的特性。KO₂以剂量依赖的方式引发快速组胺释放，而无乳酸脱氢酶（LDH）释放。KO₂诱导的释放是温度和能量依赖性的。KO₂迅速增加细胞内游离Ca²⁺浓度，同时伴有Ca²⁺摄取的显著增加。这些发现表明，细胞内Ca²⁺浓度的增加参与了KO₂诱导的组胺释放的起始过程，并且KO₂可作为O₂⁻诱导生物反应的试剂。