通过测量人淋巴细胞培养物中姐妹染色单体交换频率研究丝裂霉素C诱导的适应性反应。

Adaptive response induced by mitomycin C measuring the frequency of SCEs in human lymphocyte cultures.

作者信息

Madrigal-Bujaidar E, Cassani M, Martínez S, Morales T

机构信息

Depto. de Morfologia, Escuela Nacional de Ciencias Biológicas, IPN, México, D.F.

出版信息

Mutat Res. 1994 Oct;322(4):301-5. doi: 10.1016/0165-1218(94)90106-6.

DOI:10.1016/0165-1218(94)90106-6

PMID:7523924

Abstract

The induction of an adaptive response was obtained using mitomycin C (MMC) as both stimulating and challenging agent. Human lymphocyte cultures of two female donors were treated with 5, 10 and 20 ng/ml of MMC as conditioning doses. For the challenging treatments two different protocols were used (200 ng/ml for 4 h, and 400 ng/ml for 1 h). The scoring of sister chromatid exchanges (SCE) in the first challenging combination showed the following inhibition related with the expected SCE damage: 49.2%, 51.4%, and 36.9% for one donor, and 42.0%, 38.6%, and 34.7% for the other (corresponding to the stimulating dosages 5, 10, and 20 ng/ml, respectively). The second challenging combination gave an inhibition of 53.8%, 40.5% and 30.2% in one donor and 43.2%, 45.9% and 30.3% in the other donor.

摘要

使用丝裂霉素C（MMC）作为刺激剂和挑战剂诱导适应性反应。将两名女性供体的人淋巴细胞培养物用5、10和20 ng/ml的MMC作为预处理剂量进行处理。对于挑战处理，使用了两种不同的方案（200 ng/ml处理4小时和400 ng/ml处理1小时）。在第一个挑战组合中，姐妹染色单体交换（SCE）的评分显示出与预期的SCE损伤相关的以下抑制率：一名供体分别为49.2%、51.4%和36.9%，另一名供体分别为42.0%、38.6%和34.7%（分别对应刺激剂量5、10和20 ng/ml）。第二个挑战组合在一名供体中的抑制率为53.8%、40.5%和30.2%，在另一名供体中的抑制率为43.2%、45.9%和30.3%。