Yamada E, Kataoka H, Isozumi T, Hazama F
Department of Pathology, Shiga University of Medical Science, Otsu, Japan.
Acta Neuropathol. 1994;88(1):14-8. doi: 10.1007/BF00294354.
To investigate the role of tyrosine kinase underlying glial cell proliferation after axotomy, the localization of phosphotyrosine was studied immunohistochemically in the dorsal motor nucleus of the vagus nerve and the hypoglossal nucleus after nerve transection in adult rats. An anti-phosphotyrosine antibody weakly stained the cytoplasm of the neurons and some glial cells on the control side of both nuclei, while preferentially staining the plasma membrane of perineuronal microglial cells and neurons weakly on the severed side 2 days after axotomy and intensely between 3 and 7 days. Some of the microglial cells reacted positively with both anti-bromodeoxyuridine and anti-phosphotyrosine antibodies, suggesting that tyrosine kinase is involved in microglial cell proliferation. Proliferation of numerous microglial cells was observed in the severed nuclei between 2 and 4 days after axotomy, while only a few were detected on days 5 and 7. These findings suggest that tyrosine kinase is involved in not only the proliferation of perineuronal microglial cells but also in some retrograde neuronal reactions such as differentiation and regeneration.
为了研究轴突切断后酪氨酸激酶在胶质细胞增殖中的作用，采用免疫组织化学方法研究了成年大鼠迷走神经背运动核和舌下神经核在神经切断后磷酸酪氨酸的定位。抗磷酸酪氨酸抗体在两个核的对照侧对神经元和一些胶质细胞的细胞质进行弱染色，而在轴突切断后2天，在切断侧优先对神经元周围小胶质细胞的质膜进行弱染色，在3至7天之间进行强染色。一些小胶质细胞与抗溴脱氧尿苷和抗磷酸酪氨酸抗体均呈阳性反应，提示酪氨酸激酶参与小胶质细胞增殖。在轴突切断后2至4天，在切断的核中观察到大量小胶质细胞增殖，而在第5天和第7天仅检测到少数小胶质细胞。这些发现提示酪氨酸激酶不仅参与神经元周围小胶质细胞的增殖，还参与一些逆行性神经元反应，如分化和再生。
