Yamada E, Kataoka H, Hazama F
Department of Pathology, Shiga University of Medical Science, Otsu, Japan.
Brain Res. 1994 Mar 14;639(2):341-6. doi: 10.1016/0006-8993(94)91751-5.
Protein kinase C (PKC) and growth-associated protein-43 (GAP-43) were investigated immunohistochemically in the dorsal motor nucleus of the vagus nerve and the hypoglossal nucleus after axotomy using monoclonal antibodies against type I, II and III PKC and GAP-43. In the control side of both nuclei, anti-type I and II PKC weakly stained neuronal cell bodies, while anti-type III PKC did not show any reaction with neurons. In the axotomized side of both nuclei, anti-type II PKC antibody intensely stained affected nerve cell bodies as well as plasma membrane. Some of the severed neurons showed intensified reactions for both anti-type II PKC and anti-GAP-43 antibodies in the serial sections. These findings suggest that axotomy increases the type II PKC of the severed neurons, and type II PKC seems to phosphorylate some protein, such as GAP-43, and plays some role in the retrograde neuronal reaction.
使用抗I型、II型和III型蛋白激酶C（PKC）以及生长相关蛋白43（GAP-43）的单克隆抗体，通过免疫组织化学方法研究了迷走神经背运动核和舌下神经核在轴突切断后的PKC和GAP-43。在两个核的对照侧，抗I型和II型PKC对神经元细胞体染色较弱，而抗III型PKC与神经元无任何反应。在两个核的轴突切断侧，抗II型PKC抗体强烈染色受影响的神经细胞体以及质膜。在连续切片中，一些切断的神经元对抗II型PKC和抗GAP-43抗体均表现出增强反应。这些发现表明轴突切断增加了切断神经元的II型PKC，II型PKC似乎使某些蛋白质（如GAP-43）磷酸化，并在逆行性神经元反应中发挥一定作用。
