von Asmuth E J, Dentener M A, Ceska M, Buurman W A
Department of Surgery, Faculty II, University of Limburg, Maastricht, The Netherlands.
Eur Cytokine Netw. 1994 May-Jun;5(3):301-10.
The capacity of renal epithelial cells to produce IL-6, IL-8 and TNF was investigated. Cultures of explanted human renal cortical epithelial cells (RCEC) were established, and cytokine-release and mRNA expression by these cells were measured. IL-6, IL-8 and TNF release were measured after stimulation with IL-1 beta TNF-alpha, LPS and the phorbol esther PMA. All these agents were found to induce increased release of the three cytokines. Whilst no spontaneous TNF-release occurred, IL-6 and IL-8 were continuously released by non-stimulated RCEC cultures. IL-1 beta was the most potent trigger, enhancing both RCEC cytokine release and expression of IL-6, IL-8 and TNF mRNA. Indomethacin, budesonide, cyclosporin and FK 506 were tested for their influence on RCEC cytokine release. Only the steroid budesonide appeared to reduce both spontaneous and IL-1 beta induced cytokine release. Our data demonstrate stimulus specific release of IL-6, IL-8 and TNF by RCEC, and suggest that cytokine cell-to-cell communication may be important in regulating inflammatory processes in the kidney.
研究了肾上皮细胞产生白细胞介素-6（IL-6）、白细胞介素-8（IL-8）和肿瘤坏死因子（TNF）的能力。建立了人肾皮质上皮细胞（RCEC）外植体培养物，并检测了这些细胞的细胞因子释放和mRNA表达。在用白细胞介素-1β（IL-1β）、肿瘤坏死因子-α（TNF-α）、脂多糖（LPS）和佛波酯（PMA）刺激后，检测了IL-6、IL-8和TNF的释放。发现所有这些试剂均能诱导这三种细胞因子释放增加。虽然未发生自发性TNF释放，但未刺激的RCEC培养物持续释放IL-6和IL-8。IL-1β是最有效的触发因素，可增强RCEC细胞因子释放以及IL-6、IL-8和TNF mRNA的表达。测试了吲哚美辛、布地奈德、环孢素和他克莫司（FK 506）对RCEC细胞因子释放的影响。只有类固醇布地奈德似乎能减少自发性和IL-1β诱导的细胞因子释放。我们的数据表明RCEC可特异性释放IL-6、IL-8和TNF，并提示细胞因子的细胞间通讯可能在调节肾脏炎症过程中起重要作用。
