Burns T, Wilson M, Pearson G J
Department of Microbiology, Institute of Dental Surgery, London, UK.
J Dent. 1994 Oct;22(5):273-8. doi: 10.1016/0300-5712(94)90056-6.
Suspensions of Streptococcus mutans, S. sobrinus, Lactobacillus casei and Actinomyces viscosus were exposed to light from a gallium aluminium arsenide laser in the presence of aluminium disulphonated phthalocyanine and the numbers of survivors determined. Exposure to the laser light in the absence of the dye, or the dye in the absence of the laser light, had no significant effect on the viability of the organisms. However, a light-dose-related decrease in the viable count of all four target organisms was found on exposure to the laser light in the presence of the dye. The kills attributable to lethal photosensitization amounted to approximately 10(6) CFU in the case of each organisms. As appreciable kills were achieved within clinically convenient exposure times (30-90 s), these results imply that lethal photosensitization may be a useful technique for eliminating bacteria from carious lesions prior to restoration.
将变形链球菌、远缘链球菌、干酪乳杆菌和粘性放线菌的悬液在二磺酸铝酞菁存在的情况下，暴露于砷化镓铝激光下，并测定存活菌数。在无染料的情况下暴露于激光，或在无激光的情况下使用染料，对这些微生物的生存能力均无显著影响。然而，在有染料存在的情况下暴露于激光时，发现所有四种目标微生物的活菌数均出现与光剂量相关的减少。对于每种微生物而言，由致死性光致敏导致的杀灭量约为10(6) CFU。由于在临床方便的暴露时间（30 - 90秒）内实现了可观的杀灭效果，这些结果表明，致死性光致敏可能是一种在修复龋齿病变前清除细菌的有用技术。
