Zhang C H, Li Q H, Zhang J F, Zhou H, Zhang X B, Tang J
Labolatory of Molecular Biology, Beijing Medical University.
Sheng Li Xue Bao. 1994 Aug;46(4):347-54.
Using the Reverse Transcription (RT)-Polymerase Chain Reaction (PCR) method of Martin (1993) for semiquantitation determinating of NOS gene, it was found that NOS mRNA is not only existed in brain, but also distributed extensively in heart, kidney, lung and liver. Among of them, NOS mRNA levels were highest in the brain, followed in descending order by kidney, heart. In addition to endothelial cell, NOS gene was also highly expressed in smooth muscle cells, suggesting that they may be an important site of NOS in organism. Furthermore, NOS mRNA levels were found to decrease significantly in brain, kidney, liver and smooth muscle cell in spontaneous hypertensive rat. These data suggest that pathogeny of hypertension may be related to low expression of NOS gene in these tissues.
采用马丁（1993年）的逆转录（RT）-聚合酶链反应（PCR）方法对一氧化氮合酶（NOS）基因进行半定量测定，发现NOS信使核糖核酸（mRNA）不仅存在于大脑中，还广泛分布于心脏、肾脏、肺和肝脏中。其中，大脑中NOS mRNA水平最高，其次依次为肾脏、心脏。除内皮细胞外，NOS基因在平滑肌细胞中也高度表达，表明它们可能是机体中NOS的重要位点。此外，发现自发性高血压大鼠的大脑、肾脏、肝脏和平滑肌细胞中NOS mRNA水平显著降低。这些数据表明，高血压的发病机制可能与这些组织中NOS基因的低表达有关。
