Chen G, Zhang C, Zhu Y
Institute of Cardiovascular Research, Beijing Medical University.
Zhonghua Yi Xue Za Zhi. 1997 Nov;77(11):823-8.
To search and clone novel genes related to hypertension.
Using differential display, a RNA mapping method, we identified differentially expressed RNAs from cultured VSMCs of WKY and SHR. Down-expressed cDNAs of WKY were cloned.
A novel cDNA (rHRG-1) obtained contained 4,160 bp nucleotides and an open reading frame which encoded 551 amino acid residues. Its molecular weight was 62,644. Northern blot analysis showed that the mRNA was expressed not only in VSMCs but in various rat tissues (heart, liver, brain, lung and kidney). Besides, the expression of rHRG-1 mRNA in various tissues (heart, liver, brain and kidney) of WKY rat was higher than that in those tissues of SHR. Semi-quantitative RT-PCR analysis showed that the expression of rHRG-1 mRNA was down-regulated by treating cells with angiotensin-II, endothelin-I or interleukin-I. VSMCs proliferation and hypertension-stimulating factor, but was up-regulated when treating cells with ANF, CGRP or adrenomedullin which could inhibit the proliferation of VSMCs and attenuate hypertension. These effects could be blocked or attenuated by corresponding antagonists or antibodies.
The isolated novel rat cDNA (rHRG-1) is related to VSMCs proliferation or hypertension. It may antagonize the proliferation of VSMCs and may be involved in the formation of hypertension.
寻找并克隆与高血压相关的新基因。
采用差异显示这种RNA定位方法，我们从WKY和SHR的培养血管平滑肌细胞（VSMCs）中鉴定出差异表达的RNA。克隆了WKY中表达下调的cDNA。
获得的一个新cDNA（rHRG-1）包含4160个碱基对，有一个编码551个氨基酸残基的开放阅读框。其分子量为62644。Northern印迹分析表明，该mRNA不仅在VSMCs中表达，在大鼠的各种组织（心脏、肝脏、脑、肺和肾脏）中也有表达。此外，rHRG-1 mRNA在WKY大鼠的各种组织（心脏、肝脏、脑和肾脏）中的表达高于SHR相应组织中的表达。半定量RT-PCR分析表明，用血管紧张素-II、内皮素-I或白细胞介素-I处理细胞会使rHRG-1 mRNA的表达下调，这些物质是血管平滑肌细胞增殖和高血压刺激因子，但用心房钠尿肽、降钙素基因相关肽或肾上腺髓质素处理细胞时rHRG-1 mRNA的表达上调，这些物质可抑制血管平滑肌细胞增殖并减轻高血压。这些作用可被相应的拮抗剂或抗体阻断或减弱。
分离得到的新大鼠cDNA（rHRG-1）与血管平滑肌细胞增殖或高血压有关。它可能拮抗血管平滑肌细胞的增殖，并可能参与高血压的形成。
