针对HLA - B12的人源单克隆IgG抗体的产生与特性鉴定

Generation and characterization of a human monoclonal IgG antibody specific for HLA-B12.

作者信息

Siemoneit K, Wölpl A, Wegener S, Ernst M, Sonneborn H H, Goldmann S F

机构信息

Department of Transplantation Immunology, Red Cross Blood Bank, Ulm, FRG.

出版信息

Tissue Antigens. 1994 Jul;44(1):25-33. doi: 10.1111/j.1399-0039.1994.tb02353.x.

DOI:10.1111/j.1399-0039.1994.tb02353.x

PMID:7526486

Abstract

We describe here the generation and characterization of a human monoclonal IgG antibody (UL/F14) specific for HLA-B12. The antibody is suitable for complement-dependent lysis on lymphoblastoid cell lines and stimulated peripheral blood mononuclear cells. UL/F14 competes for antigen binding with an HLA-B12 human monoclonal IgM antibody and with a specific alloantiserum. Protein chemistry shows that the monoclonal antibody UL/F14 can precipitate solubilized HLA-B12 molecules.

摘要

我们在此描述了一种针对HLA - B12的人源单克隆IgG抗体（UL/F14）的产生及特性。该抗体适用于对淋巴母细胞系和刺激后的外周血单个核细胞进行补体依赖的细胞溶解。UL/F14可与一种HLA - B12人源单克隆IgM抗体以及一种特异性同种抗血清竞争抗原结合。蛋白质化学分析表明，单克隆抗体UL/F14能够沉淀溶解的HLA - B12分子。