两种与HLA - B27反应的人IgM单克隆抗体的特性鉴定

Characterization of two human IgM monoclonal antibodies reactive with HLA-B27.

作者信息

Wölpl A, Toubert A, Siemoneit K, Eiermann T H, Neumayer H, Goldmann S F

机构信息

Department of Transfusion Medicine, University of Ulm, Germany.

出版信息

Tissue Antigens. 1995 Oct;46(4):305-12. doi: 10.1111/j.1399-0039.1995.tb02497.x.

DOI:10.1111/j.1399-0039.1995.tb02497.x

PMID:8560449

Abstract

We describe here the generation and characterization of two human monoclonal IgM antibodies (UL-4F11 and UL-F6) reactive with HLA-B27. The monoclonal antibody (mAb) UL-4F11 is cytotoxic for peripheral mononuclear cells and, therefore, useful as typing reagent for HLA-B27 and HLA-B38. Protein chemistry showed that the mAb UL-4F11 precipitates HLA-B27 molecules. Epitope mapping analysis suggests that the amino acids 45, 67, 82 and 83 (alpha-1 domain) of the HLA-B27 sequence are necessary for mAb UL-4F11 reactivity. The mAb UL-F6 is suitable for complement dependent lysis of lymphoblastoid cell lines and stimulated peripheral blood mononuclear cells with HLA-B27 (B2701, B2702, B2703, B2705, B*2707), B13, B40 (60,61), B47 and B48 specificities. Its reactivity indicates that the amino acid valine in position 152 and glutamic acid in position 163 of the alpha-2 domain are crucial for the binding epitope.

摘要

我们在此描述了两种与HLA - B27反应的人源单克隆IgM抗体（UL - 4F11和UL - F6）的产生及特性。单克隆抗体（mAb）UL - 4F11对外周血单个核细胞具有细胞毒性，因此可用作HLA - B27和HLA - B38的分型试剂。蛋白质化学分析表明，mAb UL - 4F11能沉淀HLA - B27分子。表位定位分析表明，HLA - B27序列的第45、67、82和83位氨基酸（α - 1结构域）对于mAb UL - 4F11的反应性是必需的。mAb UL - F6适用于对具有HLA - B27（B2701、B2702、B2703、B2705、B*2707）、B13、B40（60,61）、B47和B48特异性的淋巴母细胞系和刺激的外周血单个核细胞进行补体依赖性裂解。其反应性表明，α - 2结构域第152位的缬氨酸和第163位的谷氨酸对于结合表位至关重要。