Ayers V K, Collins J K, Purdy C W
Department of Microbiology, Colorado State University, Fort Collins 80523.
Vaccine. 1994 Aug;12(10):940-6. doi: 10.1016/0264-410x(94)90038-8.
Reciprocal competition ELISA (rcELISA) was conducted to map monoclonal antibodies (mAbs) reactive with gI, gIII and gIV glycoproteins of bovine herpesvirus type 1 (BHV-1) into epitope groups. mAbs to glycoproteins gI and gIV were divided into six epitope groups each, while gIII mAbs had been previously divided into four areas. mAbs were chosen from each epitope group to compete in cELISA wih bovine sera collected during a typical regimen of vaccination and transportation from farm to auction to feedlot. The immunodominant epitopes were identified for each BHV-1 glycoprotein. With glycoprotein gI, three epitopes defined by mAbs 1F10, D9 and 4807 were the most dominant; with glycoprotein gIII epitopes defined by mAbs G2 and 1507, and with glycoprotein gIV epitopes defined by mAbs 1102, 1106, 3C1, 3402 and 3E7 showed the maximum responses. The overall cELISA responses to each glycoprotein among two vaccination groups were also compared and it was shown that cELISA responses were significantly higher for each glycoprotein in calves receiving two vaccinations, one on the farm of origin and one at auction, than in calves receiving only one vaccination at auction.
进行了相互竞争ELISA（rcELISA），以将与牛疱疹病毒1型（BHV-1）的gI、gIII和gIV糖蛋白反应的单克隆抗体（mAb）定位到表位组中。针对糖蛋白gI和gIV的单克隆抗体分别被分为六个表位组，而针对gIII的单克隆抗体先前已被分为四个区域。从每个表位组中选择单克隆抗体，在cELISA中与在从农场到拍卖场再到饲养场的典型疫苗接种和运输过程中收集的牛血清进行竞争。确定了每种BHV-1糖蛋白的免疫显性表位。对于糖蛋白gI，由单克隆抗体1F10、D9和4807定义的三个表位最为显性；对于糖蛋白gIII，由单克隆抗体G2和1507定义的表位，以及对于糖蛋白gIV，由单克隆抗体1102、1106、3C1、3402和3E7定义的表位显示出最大反应。还比较了两个疫苗接种组中对每种糖蛋白的总体cELISA反应，结果表明，在原产地农场接种一次疫苗且在拍卖场接种一次疫苗的犊牛中，每种糖蛋白的cELISA反应明显高于仅在拍卖场接种一次疫苗的犊牛。
