Tasaka K, Hamada M, Mio M
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Okayama University, Japan.
Agents Actions. 1994 Jun;41 Spec No:C26-7. doi: 10.1007/BF02007751.
Interleukin-2 (IL-2) inhibited histamine release from rat mast cells induced by compound 48/80 in a concentration-dependent manner. The inhibitory effect of IL-2 on histamine release was also dependent on the length of the incubation period; the maximum inhibition was achieved at 8 h after IL-2 addition. Furthermore, IL-2 inhibited not only IP3 production but also 45Ca uptake in mast cells stimulated by compound 48/80. Since IL-2 enhanced [3H]-leucine uptake into mast cells, this suggests that protein synthesis may be related in some way with the inhibition of histamine release. IL-2 treatment augmented the synthesis of a protein having a molecular weight of approximately 35 kDa. From Western blotting analysis, it became clear that the production of lipocortin-I was augmented in rat mast cells by IL-2 treatment. The present study shows that IL-2 induces the synthesis of lipocortin-I in mast cells and that lipocortin-I may play some role in inhibiting histamine release from mast cells.
白细胞介素-2（IL-2）以浓度依赖的方式抑制化合物48/80诱导的大鼠肥大细胞组胺释放。IL-2对组胺释放的抑制作用还取决于孵育时间；在添加IL-2后8小时达到最大抑制。此外，IL-2不仅抑制化合物48/80刺激的肥大细胞中IP3的产生，还抑制45Ca的摄取。由于IL-2增强了[3H]-亮氨酸进入肥大细胞的摄取，这表明蛋白质合成可能在某种程度上与组胺释放的抑制有关。IL-2处理增加了一种分子量约为35 kDa的蛋白质的合成。从蛋白质印迹分析可知，IL-2处理使大鼠肥大细胞中脂皮质素-I的产生增加。本研究表明，IL-2诱导肥大细胞中脂皮质素-I的合成，并且脂皮质素-I可能在抑制肥大细胞组胺释放中发挥某种作用。
