Wang H W, Lin J K, Wang J Y
Department of Otolaryngology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, Republic of China.
Eur Arch Otorhinolaryngol. 1994;251(5):283-6. doi: 10.1007/BF00181886.
The glyoxylic catecholaminergic histofluorescence method was employed on the mucosa of the rat's eustachian tube (ET) in order to study the sympathetic innervation present. One percent neutral red was used as counterstain. Many noradrenergic fibers were demonstrable around blood vessels, glands and submucosa of the ET, but not in the epithelium. In a group of rats following neurectomy, the superior cervical ganglia (SCG) were removed unilaterally or bilaterally. Changes in sympathetic innervation of the ET were examined 14 days after SCG ganglionectomy. In those animals after unilateral SCG ganglionectomy, no noradrenergic histofluorescence was found in the ipsilateral ET, although some scant fluorescence could be detected in the tube's nasopharyngeal (NP) orifice. However, no noradrenergic histofluorescence could be observed in animals bilateral SCG ganglionectomies. Our results indicate that sympathetic innervation of the ET in the rat originates in the SCG, with some cross-innervation of sympathetic fibers occurring in the tube's NP orifice.
为了研究大鼠咽鼓管（ET）黏膜中存在的交感神经支配，采用了乙醛酸儿茶酚胺能组织荧光法。使用1%中性红作为复染剂。在ET的血管、腺体和黏膜下层周围可显示出许多去甲肾上腺素能纤维，但上皮中没有。在一组接受神经切除术的大鼠中，单侧或双侧切除颈上神经节（SCG）。在SCG神经节切除术后14天检查ET交感神经支配的变化。在单侧SCG神经节切除术后的那些动物中，同侧ET中未发现去甲肾上腺素能组织荧光，尽管在该管的鼻咽（NP）开口处可检测到一些微弱的荧光。然而，在双侧SCG神经节切除的动物中未观察到去甲肾上腺素能组织荧光。我们的结果表明，大鼠ET的交感神经支配起源于SCG，在该管的NP开口处存在一些交感神经纤维的交叉支配。
