Okada H, Yoshida J, Seo H, Wakabayashi T, Sugita K, Hagiwara M
Department of Neurosurgery, Nagoya University School of Medicine, Japan.
Cancer Immunol Immunother. 1994 Nov;39(5):313-7. doi: 10.1007/BF01519984.
We raised an anti-glioma monoclonal antibody, named G-22, that specifically recognizes a human glioma-associated surface antigen. Proven to be useful for target imaging of malignant gliomas after radioisotope labeling and cerebrospinal fluid diagnosis by enzyme-linked immunospecific assay, G-22 was found to immunoprecipitate an 85-kDa glycoprotein of the human glioma U-251MG cell. We purified this antigen by G-22-coupled cyanogenbromide-activated Sepharose affinity chromatography, and sequence analysis demonstrated that the 54 amino acid residues were identical to positions 55-108 of human CD44. The results show that the smallest spliced form (85 kDa) of CD44 is strongly expressed in glioma cells.
我们制备了一种抗胶质瘤单克隆抗体，命名为G-22，它能特异性识别一种人类胶质瘤相关表面抗原。经放射性同位素标记后，G-22被证明可用于恶性胶质瘤的靶向成像，并通过酶联免疫特异性测定进行脑脊液诊断。研究发现，G-22能免疫沉淀人胶质瘤U-251MG细胞的一种85kDa糖蛋白。我们通过G-22偶联的溴化氰活化琼脂糖亲和层析纯化了该抗原，序列分析表明，54个氨基酸残基与人CD44的55-108位相同。结果表明，CD44最小的剪接形式（85kDa）在胶质瘤细胞中高表达。
