KIF1B，一种用于线粒体运输的新型微管正端定向单体运动蛋白。

KIF1B, a novel microtubule plus end-directed monomeric motor protein for transport of mitochondria.

作者信息

Nangaku M, Sato-Yoshitake R, Okada Y, Noda Y, Takemura R, Yamazaki H, Hirokawa N

机构信息

Department of Anatomy and Cell Biology School of Medicine, University of Tokyo, Japan.

出版信息

Cell. 1994 Dec 30;79(7):1209-20. doi: 10.1016/0092-8674(94)90012-4.

DOI:10.1016/0092-8674(94)90012-4

PMID:7528108

Abstract

To further elucidate the mechanism of organelle transport, we cloned a novel member of the mouse kinesin superfamily, KIF1B. This N-terminal-type motor protein is expressed ubiquitously in various kinds of tissues. In situ hybridization revealed that KIF1B is expressed abundantly in differentiated nerve cells. Interestingly, K1F1B works as a monomer, having a microtubule plus end-directed motility. Our rotary shadowing electron microscopy revealed mostly single globular structures. Immunocytochemically, KIF1B was colocalized with mitochondria in vivo. Furthermore, a subcellular fractionation study showed that KIF1B was concentrated in the mitochondrial fraction, and purified K1F1B could transport mitochondria along microtubules in vitro. These data strongly suggested that KIF1B works as a monomeric motor for anterograde transport of mitochondria.

摘要

为了进一步阐明细胞器运输的机制，我们克隆了小鼠驱动蛋白超家族的一个新成员KIF1B。这种N端型运动蛋白在各种组织中普遍表达。原位杂交显示KIF1B在分化的神经细胞中大量表达。有趣的是，K1F1B作为单体发挥作用，具有微管正端定向运动性。我们的旋转阴影电子显微镜显示大多为单个球状结构。免疫细胞化学研究表明，KIF1B在体内与线粒体共定位。此外，亚细胞分级分离研究表明，KIF1B集中在线粒体部分，并且纯化的K1F1B能够在体外沿着微管运输线粒体。这些数据有力地表明，KIF1B作为单体马达蛋白负责线粒体的顺行运输。