Ohkawa K, Hayashi N, Yuki N, Hagiwara H, Kato M, Yamamoto K, Eguchi H, Fusamoto H, Masuzawa M, Kamada T
First Department of Medicine, Osaka University School of Medicine, Japan.
J Hepatol. 1994 Oct;21(4):509-14. doi: 10.1016/s0168-8278(94)80094-4.
We assessed hepatitis C virus infection in 156 chronic hepatitis B patients using second-generation hepatitis C virus antibody (anti-HCV). Active virus replication was further investigated in anti-HCV-positive cases by means of polymerase chain reaction assay for the detection of serum hepatitis C virus RNA. Anti-HCV prevalence was higher in patients negative for hepatitis B e antigen (HBeAg) (10/48, 21%) than in HBeAg-positive patients (10/108, 9%) (p < 0.05), and the reactivity (cut-off index) in anti-HCV enzyme-linked immunosorbent assay of the positive cases was significantly higher in HBeAg-negative patients (4.1 +/- 0.1) than in -positive ones (3.6 +/- 0.6) (p < 0.05). The prevalence of hepatitis C virus RNA in anti-HCV-positive cases was also higher in the HBeAg-negative group (9/10, 90%) than in the -positive group (3/10, 30%) (p < 0.01). Viremia was found in association with high reactivity in anti-HCV ELISA (cut-off index > 3.5) in both groups. Nine (90%) of 10 such cases were viremic in the HBeAg-negative group compared with three (43%) of seven in the HBeAg-positive group (p < 0.05). These results suggest that hepatitis C virus replication may be influenced by hepatitis B virus replicative states, indicating possible interference between hepatitis B and C viruses.
我们使用第二代丙型肝炎病毒抗体（抗-HCV）对156例慢性乙型肝炎患者进行了丙型肝炎病毒感染评估。对于抗-HCV阳性病例，通过聚合酶链反应检测血清丙型肝炎病毒RNA进一步研究病毒的活跃复制情况。乙型肝炎e抗原（HBeAg）阴性患者的抗-HCV流行率（10/48，21%）高于HBeAg阳性患者（10/108，9%）（p<0.05），并且抗-HCV酶联免疫吸附试验中阳性病例的反应性（临界指数）在HBeAg阴性患者（4.1±0.1）中显著高于阳性患者（3.6±0.6）（p<0.05）。抗-HCV阳性病例中丙型肝炎病毒RNA的流行率在HBeAg阴性组（9/10，90%）也高于阳性组（3/10，30%）（p<0.01）。在两组中均发现病毒血症与抗-HCV ELISA中的高反应性（临界指数>3.5）相关。在HBeAg阴性组的10例此类病例中有9例（90%）存在病毒血症，而在HBeAg阳性组的7例中有3例（43%）（p<0.05）。这些结果表明丙型肝炎病毒复制可能受乙型肝炎病毒复制状态的影响，提示乙型和丙型肝炎病毒之间可能存在相互干扰。
