Rock G, Chauhan K, Jamieson G A, Tandon N N
Department of Vascular Surgery, Ottawa Civic Hospital, Ontario, Canada.
Br J Haematol. 1994 Dec;88(4):878-80. doi: 10.1111/j.1365-2141.1994.tb05131.x.
Six patients with lupus anticoagulant with thrombotic complications, but not exhibiting systemic lupus erythematosus, demonstrated the presence in their plasma of antibodies directed against platelet antigens which were not detectable in two patients presenting with lupus anticoagulant but without thrombotic complications. Protein blotting of separated normal platelet proteins against patient plasma gave up to 18 bands of varying intensity indicative of multiple antiplatelet antibodies; one of these antibodies recognized a component with a mobility identical with CD36 (GPIV; m.w. 88,000) in 4/6 cases. Antibodies to CD36 and one or two other components were identified in 5/6 cases by immunoprecipitation from 125I-labelled control platelets and 6/6 by dot blots against purified CD36. These results suggest that antiplatelet antibodies and, specifically, anti CD36 antibodies, occur frequently in the plasma of patients presenting with lupus anticoagulant and thrombotic complications.
六名患有狼疮抗凝物且有血栓形成并发症但未表现出系统性红斑狼疮的患者，其血浆中显示出针对血小板抗原的抗体，而在两名患有狼疮抗凝物但无血栓形成并发症的患者中未检测到这些抗体。用患者血浆对分离出的正常血小板蛋白进行蛋白质印迹分析，得到多达18条强度各异的条带，表明存在多种抗血小板抗体；其中一种抗体在4/6的病例中识别出一种迁移率与CD36（GPIV；分子量88,000）相同的成分。通过从125I标记的对照血小板中进行免疫沉淀，在5/6的病例中鉴定出针对CD36和一两种其他成分的抗体，通过针对纯化CD36的斑点印迹法在6/6的病例中鉴定出这些抗体。这些结果表明，抗血小板抗体，特别是抗CD36抗体，在患有狼疮抗凝物和血栓形成并发症的患者血浆中频繁出现。
