Lubaroff D M, Reynolds C W, Culp D A
Trans Am Assoc Genitourin Surg. 1978;70:60-5.
We report herein on the destruction by cryosurgery of the Dunning R3327 adenocarcinoma grown in the prostate. Tumors less than 1,000 mm.3 were destroyed by a single freezing procedure within 2 to 4 weeks, whereas tumors greater than 1,000 mm.3 could not be destroyed completely. We also have been able to demonstrate antibody and cellular immune responses to antigens on the R3327 cells. Species, organ and tumor specific antigens were identified by xenogeneic antiserum produced in the rabbit, while major and minor antigens were identified by alloantisera produced in the rat. Tumor antigens were characterized by a variety of in vitro and in vivo immune responses. In addition to the rabbit xenoantisera used in cytotoxicity assays, mixed lymphocyte tumor interaction produced a demonstrable in vitro immune response, lymphocytes from rats immunized with tumor cells were cytotoxic to radiolabeled tumor cells in culture, rats immunized with tumor cells in adjuvant demonstrated protection against subsequent challenge and immunological stimulation of lymph nodes draining the site of tumor cell inoculation was demonstrated by an increase in lymphocyte trapping. Clearing, the R3327 tumor system in the rat is suitable for immunological studies of prostatic cancer.
我们在此报告通过冷冻手术对生长于前列腺的邓宁R3327腺癌的破坏情况。体积小于1000立方毫米的肿瘤在2至4周内通过单次冷冻程序被破坏，而体积大于1000立方毫米的肿瘤则无法被完全破坏。我们还能够证明对R3327细胞上抗原的抗体和细胞免疫反应。通过在兔体内产生的异种抗血清鉴定出物种、器官和肿瘤特异性抗原，而通过在大鼠体内产生的同种抗血清鉴定出主要和次要抗原。肿瘤抗原通过多种体外和体内免疫反应进行表征。除了用于细胞毒性测定的兔异种抗血清外，混合淋巴细胞肿瘤相互作用产生了可证明的体外免疫反应，用肿瘤细胞免疫的大鼠的淋巴细胞对培养中的放射性标记肿瘤细胞具有细胞毒性，用佐剂中的肿瘤细胞免疫的大鼠表现出对后续攻击的保护作用，并且通过淋巴细胞捕获增加证明了对肿瘤细胞接种部位引流淋巴结的免疫刺激。总之，大鼠中的R3327肿瘤系统适用于前列腺癌的免疫学研究。
