Hagensee M E, Carter J J, Wipf G C, Galloway D A
Fred Hutchinson Cancer Research Center, Seattle, Washington 98104-2029.
Virology. 1995 Jan 10;206(1):174-82. doi: 10.1016/s0042-6822(95)80032-8.
To assess the utility of vaccinia virus recombinants in the development of an immune response against HPV capsid antigens, 5-week-old C57B16 female mice were administered either purified HPV 1 capsids produced by a vaccinia virus recombinant or the recombinant vaccinia virus itself. Animals were boosted at Week 4 with either agent. Mice developed a serum IgG antibody response in all the administration protocols that was directed mainly against native L1 epitopes. Mice injected initially with the vaccinia virus recombinant and boosted with purified capsids had a higher titer antibody response (P = 0.024) with more mice responding to a greater extent. All mice produced a serum IgM response that preceded the IgG response by approximately 2 weeks and lasted 1-3 weeks. The IgM response was directed against native L1 epitopes. Although no serum IgA was detected, IgA could be detected in vaginal secretions of mice that were immunized or boosted with the vaccinia virus vector. These results indicate that an extensive humoral immune response to HPV can be elicited using vaccinia virus recombinants.
为评估痘苗病毒重组体在引发针对人乳头瘤病毒（HPV）衣壳抗原的免疫反应中的效用，给5周龄的C57B16雌性小鼠接种由痘苗病毒重组体产生的纯化HPV 1衣壳或痘苗病毒重组体本身。在第4周时用任一制剂对动物进行加强免疫。在所有给药方案中，小鼠均产生了血清IgG抗体反应，该反应主要针对天然L1表位。最初注射痘苗病毒重组体并用纯化衣壳进行加强免疫的小鼠具有更高滴度的抗体反应（P = 0.024），更多小鼠有更大程度的反应。所有小鼠均产生血清IgM反应，该反应比IgG反应提前约2周出现，并持续1 - 3周。IgM反应针对天然L1表位。虽然未检测到血清IgA，但在用痘苗病毒载体免疫或加强免疫的小鼠的阴道分泌物中可检测到IgA。这些结果表明，使用痘苗病毒重组体可引发对HPV的广泛体液免疫反应。
