Rose R C, Lane C, Wilson S, Suzich J A, Rybicki E, Williamson A L
University of Rochester School of Medicine and Dentistry, Department of Medicine, NY 14642, USA.
Vaccine. 1999 Apr 23;17(17):2129-35. doi: 10.1016/s0264-410x(98)00484-8.
To assess whether oral vaccination against human papillomavirus (HPV) may be feasible, we administered HPV virus-like particles (VLPs) to mice by gavage. Enzyme-linked immunosorbent assay (ELISA) results indicated that serum anti-VLP immunoglobulin G (IgG) and IgA antibodies were induced after oral vaccination, and these responses demonstrated antigenic specificities that were conformationally dependent and restricted according to HPV genotype. Importantly, orally induced postimmune sera were found to neutralize HPV-11 virions in vitro. These results indicated that the VLPs were antigenically stable in the environment of the gastrointestinal tract and were able to engage in potentially useful immune system interactions. These findings support the concept of oral vaccination against anogenital HPV disease, and suggest the possibility that this may be a useful approach to the immunization of large populations against cervical cancer and other HPV associated diseases.
为评估口服人乳头瘤病毒（HPV）疫苗是否可行，我们通过灌胃法给小鼠接种HPV病毒样颗粒（VLPs）。酶联免疫吸附测定（ELISA）结果表明，口服疫苗后诱导产生了血清抗VLP免疫球蛋白G（IgG）和IgA抗体，这些反应显示出抗原特异性，该特异性取决于构象并根据HPV基因型受到限制。重要的是，发现口服诱导的免疫后血清在体外可中和HPV - 11病毒粒子。这些结果表明，VLPs在胃肠道环境中抗原稳定，并且能够参与潜在有用的免疫系统相互作用。这些发现支持针对肛门生殖器HPV疾病进行口服疫苗接种的概念，并表明这可能是一种为大量人群接种宫颈癌和其他HPV相关疾病疫苗的有用方法。
