Conn C A, McClellan J L, Maassab H F, Smitka C W, Majde J A, Kluger M J
Institute for Basic and Applied Medical Research, Lovelace Institutes, Albuquerque, New Mexico 87108.
Am J Physiol. 1995 Jan;268(1 Pt 2):R78-84. doi: 10.1152/ajpregu.1995.268.1.R78.
This study characterized selected aspects of the acute phase response after intranasal inoculation of mice with two doses of mouse-adapted influenza virus differing in lethality. Mice given 140 plaque-forming units (PFU) of virus (58% survival) gradually decreased food and water intake to nearly zero over 6 days; survivors then slowly increased intakes. Declines in these behaviors were parallel to decreases in body temperature and general locomotor activity and were associated with elevated activities of interleukin-6 (IL-6), tumor necrosis factor-alpha, and interferons in lung lavage fluid. Circulating levels of these cytokines were not increased. After 55,000 PFU of virus (100% mortality), food and water intake fell to near zero within 48 h, temperature and locomotor activity decreased significantly, and activities of IL-1 and IL-6 were elevated in lung lavage fluid. These data show that cytokine activities in the lungs are elevated in a time frame that supports the hypothesis that cytokines could mediate behavioral and physiological changes in mice during acute influenza infections.
本研究对用两剂致死性不同的小鼠适应型流感病毒经鼻接种小鼠后的急性期反应的选定方面进行了表征。接种140个空斑形成单位（PFU）病毒的小鼠（58%存活）在6天内食物和水摄入量逐渐减少至几乎为零；幸存者随后摄入量缓慢增加。这些行为的下降与体温和总体运动活动的降低平行，并且与肺灌洗液中白细胞介素-6（IL-6）、肿瘤坏死因子-α和干扰素的活性升高有关。这些细胞因子的循环水平没有增加。接种55000 PFU病毒后（100%死亡率），食物和水摄入量在48小时内降至接近零，体温和运动活动显著下降，肺灌洗液中IL-1和IL-6的活性升高。这些数据表明，肺部细胞因子活性在一个时间范围内升高，这支持了细胞因子可能在急性流感感染期间介导小鼠行为和生理变化的假说。
