Van Reeth K, Nauwynck H, Pensaert M
Laboratory of Veterinary Virology, Faculty of Veterinary Medicine, University of Ghent, Merelbeke, Belgium.
J Infect Dis. 1998 Apr;177(4):1076-9. doi: 10.1086/517398.
Biologically active interferon-alpha, tumor necrosis factor-alpha (TNF-alpha), and interleukin-1 (IL-1) were detected in bronchoalveolar lavage (BAL) fluids of 3-week-old cesarian-derived colostrum-deprived pigs inoculated with H1N1 influenza virus. Cytokine titers and lung virus titers were significantly higher 18-24 h after inoculation than at 48-72 h after inoculation in all 4 litters of pigs examined. All three cytokines were positively correlated with a 3- to 4-fold increase in BAL cell numbers (P < .036) and with a drastic neutrophil infiltration (24%-77% of BAL cells vs. 0-1.5% in controls) (P < .001). In addition, cytokine production coincided with the onset of general and respiratory symptoms of influenza and with the development of a necrotizing bronchopneumonia. This study is the first demonstration of TNF-alpha and IL-1 in BAL fluids of a natural influenza virus host. It documents that pigs may be a highly valuable experimental model in human influenza virus pneumonia.
在接种H1N1流感病毒的3周龄剖腹产初乳缺乏仔猪的支气管肺泡灌洗（BAL）液中检测到具有生物活性的α干扰素、肿瘤坏死因子-α（TNF-α）和白细胞介素-1（IL-1）。在所检查的所有4窝仔猪中，接种后18 - 24小时的细胞因子滴度和肺病毒滴度显著高于接种后48 - 72小时。所有这三种细胞因子均与BAL细胞数量增加3至4倍呈正相关（P < 0.036），并与严重的中性粒细胞浸润（BAL细胞的24% - 77%，而对照组为0 - 1.5%）呈正相关（P < 0.001）。此外，细胞因子的产生与流感的全身和呼吸道症状的出现以及坏死性支气管肺炎的发展同时发生。本研究首次在天然流感病毒宿主的BAL液中证实了TNF-α和IL-1的存在。它证明猪可能是人类流感病毒肺炎中非常有价值的实验模型。
