Gaia V, Poloni C, Peduzzi R
Laboratoire d'écologie microbienne, Université de Genève, Switzerland.
Eur J Epidemiol. 1994 Jun;10(3):303-6. doi: 10.1007/BF01719354.
The ribotyping method, adapted to the strains of Legionella pneumophila in our possession, was tested in two separate cases of legionellosis and in the associated finding of Legionella pneumophila in the water, from different sources, with which these patients had come into contact. Determination of the serogroup enabled us to carry out a preliminary analysis of the strains, which was then confirmed by application of the ribotyping procedure: the ribosomal profile of the strains found in the two patients correspond to that of the strains isolated from the water with which they had come into contact. These results provide important information concerning the probable sources of infection involved in these two cases of Legionnaires' disease. We consider ribotyping to be a very useful tool, which is easy and simple to perform and is applicable to the Legionella genus as the method of choice for epidemiological studies.
我们采用适合手头嗜肺军团菌菌株的核糖体分型方法,在两例军团病个案以及这些患者接触过的不同水源中嗜肺军团菌的相关检测中进行了测试。血清群的测定使我们能够对菌株进行初步分析,随后通过核糖体分型程序得到确认:在两名患者身上发现的菌株核糖体图谱与从他们接触过的水中分离出的菌株图谱相符。这些结果为这两例军团病可能的感染源提供了重要信息。我们认为核糖体分型是一种非常有用的工具,操作简便,适用于军团菌属,是流行病学研究的首选方法。