Deen S, Ball R Y
Department of Histopathology/Cytopathology, Norfolk & Norwich Hospital, UK.
Histopathology. 1994 Nov;25(5):475-81. doi: 10.1111/j.1365-2559.1994.tb00010.x.
Immunohistochemical studies to localise collagen IV, laminin, fibronectin and tenascin were undertaken in 59 bladder biopsies. These were non-neoplastic or contained foci of flat dysplasia or of transitional cell neoplasia. Collagen IV and laminin were reliably demonstrated in basement membranes but focal loss of reactivity for collagen IV was found in the urothelial basement membrane in some cases of inflammation, dysplasia, and non-invasive papillary transitional cell tumour. Basement membranes were rarely demonstrated around invasive transitional cell carcinomas. Fibronectin and tenascin were found in the sub-urothelial lamina propria and in some urothelial basement membranes. The stroma of invasive tumours reacted strongly for both. Increased numbers of sub-urothelial capillaries were present in association with some cases of cystitis, flat dysplasia or non-invasive transitional cell neoplasia suggesting an angiogenic stimulus in these circumstances. Immunohistochemical staining of basement membrane components is unlikely to be of value in the routine histopathological assessment of transitional cell neoplasms of the bladder.
对59例膀胱活检组织进行了免疫组织化学研究，以定位IV型胶原、层粘连蛋白、纤连蛋白和腱生蛋白。这些组织为非肿瘤性或含有扁平发育异常灶或移行细胞肿瘤灶。IV型胶原和层粘连蛋白在基底膜中得到可靠证实，但在某些炎症、发育异常和非浸润性乳头状移行细胞肿瘤病例中，尿路上皮基底膜中发现IV型胶原反应性局灶性丧失。浸润性移行细胞癌周围很少显示基底膜。纤连蛋白和腱生蛋白见于尿路上皮下固有层和一些尿路上皮基底膜。浸润性肿瘤的间质对两者均有强烈反应。在一些膀胱炎、扁平发育异常或非浸润性移行细胞肿瘤病例中，尿路上皮下毛细血管数量增加，提示在这些情况下存在血管生成刺激。基底膜成分的免疫组织化学染色在膀胱移行细胞肿瘤的常规组织病理学评估中不太可能有价值。
